Rat D- Lactate Dehydrogenase, D- LDH ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Probable D-lactate dehydrogenase, mitochondrial(DLD,Lactate dehydrogenase D), LDHD,lactate dehydrogenase D; DLD; D-lactate dehydrogenase

Search name

Rat Probable D-lactate dehydrogenase mitochondrial ELISA KIT ,Rat DLD ELISA KIT ,Rat Lactate dehydrogenase D ELISA KIT ,Rat LDHD ELISA KIT ,Rat lactate dehydrogenase D ELISA KIT ,Rat DLD ELISA KIT ,Rat D-lactate dehydrogenase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat probable d-lactate dehydrogenase, mitochondrial,DLD concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to DLD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DLD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DLD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DLD in the samples is then determined by comparing the O.D. of the samples to the standard curve.