Rat Dystrophin, DMD ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Dmd,Dystrophin,BMD; CMD3B; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; MRX85

Search name

Rat Dmd ELISA KIT ,Rat Dystrophin ELISA KIT ,Rat BMD ELISA KIT ,Rat CMD3B ELISA KIT ,Rat DXS142 ELISA KIT ,Rat DXS164 ELISA KIT ,Rat DXS206 ELISA KIT ,Rat DXS230 ELISA KIT ,Rat DXS239 ELISA KIT ,Rat DXS268 ELISA KIT ,Rat DXS269 ELISA KIT ,Rat DXS270 ELISA KIT ,Rat DXS272 ELISA KIT ,Rat MRX85 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Dystrophin concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dystrophin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Dystrophin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dystrophin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dystrophin in the samples is then determined by comparing the O.D. of the samples to the standard curve.