Rat E3 ubiquitin- protein ligase HUWE1, HUWE1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Huwe1,Kiaa0312,Ureb1,E3 ubiquitin-protein ligase HUWE1,ARF-BP1,HECTH9,HSPC272,Ib772,LASU1,MULE,URE-B1,UREB1,ARF-binding protein 1,BJ-HCC-24 tumor antigen,HECT domain protein LASU1,Mcl-1 ubiquitin ligase E3,URE-binding protein 1,homologous to E6AP carboxyl terminus homologous protein 9,large structure of UREB1,upstream regulatory element-binding protein 1,HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase

Search name

Rat Huwe1 ELISA KIT ,Rat Kiaa0312 ELISA KIT ,Rat Ureb1 ELISA KIT ,Rat E3 ubiquitin-protein ligase HUWE1 ELISA KIT ,Rat ARF-BP1 ELISA KIT ,Rat HECTH9 ELISA KIT ,Rat HSPC272 ELISA KIT ,Rat Ib772 ELISA KIT ,Rat LASU1 ELISA KIT ,Rat MULE ELISA KIT ,Rat URE-B1 ELISA KIT ,Rat UREB1 ELISA KIT ,Rat ARF-binding protein 1 ELISA KIT ,Rat BJ-HCC-24 tumor antigen ELISA KIT ,Rat HECT domain protein LASU1 ELISA KIT ,Rat Mcl-1 ubiquitin ligase E3 ELISA KIT ,Rat URE-binding protein 1 ELISA KIT ,Rat homologous to E6AP carboxyl terminus homologous protein 9 ELISA KIT ,Rat large structure of UREB1 ELISA KIT ,Rat upstream regulatory element-binding protein 1 ELISA KIT ,Rat HECT ELISA KIT ,Rat  UBA and WWE domain containing 1 ELISA KIT ,Rat  E3 ubiquitin protein ligase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat E3 ubiquitin-protein ligase HUWE1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to E3 ubiquitin-protein ligase HUWE1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for E3 ubiquitin-protein ligase HUWE1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain E3 ubiquitin-protein ligase HUWE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of E3 ubiquitin-protein ligase HUWE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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