Rat Endothelial cell-specific molecule 1,ESM1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Endothelial cell-specific molecule ,ESM-1, ESM1

Search name

Rat ESM1 ELISA Kit, Rat Endothelial cell-specific molecule 1 ELISA Kit, Rat ESM-1 ELISA Kit, ESM1 ELISA Kit, Endothelial cell-specific molecule 1 ELISA Kit, ESM-1 ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat endothelial cell-specific molecule 1,ESM-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to ESM-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ESM-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ESM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ESM-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.