Rat Erythropoietin, EPO ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Erythropoietin,;

Search name
Rat Erythropoietin ELISA Kit, Rat EPO ELISA Kit, Erythropoietin ELISA Kit, EPO ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat erythropoietin,Erythropoietin concentrations in serum,
Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythropoietin. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Erythropoietin and
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is
added to each well. Only those wells that contain Erythropoietin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a
change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Erythropoietin in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

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