Rat Fibrinogen Gamma, FGg ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE

BEGINNING!

Synonyms

FGG, Fibrinogen gamma ,fibrinogen gamma chain; fibrinogen, gamma polypeptide,

Search name

Rat FGG ELISA KIT ,Rat Fibrinogen gamma ELISA KIT ,Rat fibrinogen gamma chain ELISA KIT ,Rat fibrinogen ELISA KIT ,Rat gamma polypeptide ELISA KIT, Rat PRO2061 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat fibrinogen gamma chain concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen gamma chain. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Fibrinogen gamma chain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Fibrinogen gamma chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Fibrinogen gamma chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.