Rat GSR ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  GSR

Protein Name  GSR mitochondrial

Rat GSR ELISA KIT  Detection Range 

Alternative Name

Rat GSR ELISA KIT ,Rat GSR mitochondrial mitochondrial ELISA KIT ,Rat HEL-75 ELISA KIT ,Rat HEL-S-122m ELISA KIT ,Rat GRase ELISA KIT ,Rat epididymis luminal protein 75 ELISA KIT ,Rat epididymis secretory sperm binding protein Li 122m ELISA KIT ,Rat glutathione S-reductase ELISA KIT ,Rat glutathione-disulfide reductase ELISA KIT ,Rat GR ELISA KIT ,Rat GSR ELISA KIT

Intended use

Rat GSR ELISA KIT allows for the in vitro quantitative determination of GSR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Rat GSR ELISA KIT has been pre-coated with an GSR antibody specific to GSR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GSR and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GSR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GSR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20