Rat Glutathione S- transferase P, GSTP1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Glutathione S-transferase P,GST class-pi,GSTP1-1,GSTP1,GSTP1-1; DFN7; FAEES3; GST3; GSTP; HEL-S-22; GST class-pi; deafness, X-linked 7; epididymis secretory protein Li 22; fatty acid ethyl ester synthase III; glutathione S-transferase P; glutathione S-transferase pi 1, P,GST class-pi,Chain 7,GST 7-7,
Search name
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Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Glutathione S-transferase P concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S-transferase P. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glutathione S-transferase P and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glutathione S-transferase P, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glutathione S-transferase P in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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