Rat Indoleamine 2,3-dioxygenase 1,IDO1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

IDO1,IDO-1; IDO; INDO; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine-pyrrole 2,3-dioxygenase; indoleamine 2,3-dioxygenase 1

Search name

Rat IDO1 ELISA KIT, Rat IDO-1 ELISA KIT Rat,IDO ELISA KIT, Rat INDO ELISA KIT,Rat indolamine 2,3 dioxygenase ELISA KIT, Rat indole 2,3-dioxygenase ELISA KIT, Rat indoleamine-pyrrole 2,3-dioxygenase ELISA KIT, Rat indoleamine 2,3-dioxygenase 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat indoleamine 2,3-dioxygenase 1,IDO1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to IDO1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IDO1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IDO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IDO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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