Rat Kallikrein- 1, Ngfg ELISA KIT

Product Name:Rat Kallikrein- 1, Ngfg ELISA KIT
Packing:96T

Catalog No.:ELI-15892r

Gene Name:Ngfg

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Rat Kallikrein- 1

Alternative Name:Ngfg,Rat Kallikrein- 1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Rat Kallikrein- 1, Ngfg ELISA KIT allows for the in vitro quantitative determination ofRat Kallikrein- 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Rat Kallikrein- 1, Ngfg ELISA KIT has been pre-coated with an Ngfg antibody specific to Rat Kallikrein- 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rat Kallikrein- 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Kallikrein- 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rat Kallikrein- 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!