Rat Krueppel- like factor 10, KLF10 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
KLF10,EGR-alpha; EGRA; TIEG; TIEG1; Krueppel-like factor 10; TGFB-inducible early growth response protein 1; early growth response-alpha; transforming growth factor-beta-inducible early growth response protein 1; zinc finger transcription factor TIEG; Kruppel-like factor 10, TIEG-1
Search name
Rat KLF10 ELISA KIT ,Rat EGR-alpha ELISA KIT ,Rat EGRA ELISA KIT ,Rat TIEG ELISA KIT ,Rat TIEG1 ELISA KIT ,Rat Krueppel-like factor 10 ELISA KIT ,Rat TGFB-inducible early growth response protein 1 ELISA KIT ,Rat early growth response-alpha ELISA KIT ,Rat transforming growth factor-beta-inducible early growth response protein 1 ELISA KIT ,Rat zinc finger transcription factor TIEG ELISA KIT ,Rat Kruppel-like factor 10 ELISA KIT ,Rat TIEG-1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Krueppel-like factor 10 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Krueppel-like factor 10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Krueppel-like factor 10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Krueppel-like factor 10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Krueppel-like factor 10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.