Rat Major prion protein,PRNP ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Major prion protein,PRNP,PrP,PrP33-35C,ASCR,PrP27-30, Major prion protein,prion protein,ASCR; AltPrP; CD230; CJD; GSS; KURU; PRIP; PrP; PrP27-30; PrP33-35C; PrPc; p27-30; CD230 antigen; prion-related protein

Search name

Rat Major prion protein ELISA KIT ,Rat PRNP ELISA KIT ,Rat PrP ELISA KIT ,Rat PrP33-35C ELISA KIT ,Rat ASCR ELISA KIT ,Rat PrP27-30 ELISA KIT ,Rat  Major prion protein ELISA KIT ,Rat prion protein ELISA KIT ,Rat ASCR ELISA KIT ,Rat AltPrP ELISA KIT ,Rat CD230 ELISA KIT ,Rat CJD ELISA KIT ,Rat GSS ELISA KIT ,Rat KURU ELISA KIT ,Rat PRIP ELISA KIT ,Rat PrP ELISA KIT ,Rat PrP27-30 ELISA KIT ,Rat PrP33-35C ELISA KIT ,Rat PrPc ELISA KIT ,Rat p27-30 ELISA KIT ,Rat CD230 antigen ELISA KIT ,Rat prion-related protein ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat major prion protein ,Rat PrP concentrations in serum ,Rat  Plasma ,Rat  tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PrP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PrP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PrP ,Rat  biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PrP in the samples is then determined by comparing the O.D. of the samples to the standard curve.