Rat Malignant T cell- amplified sequence 1, MCTS1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Malignant T cell-amplified sequence 1(MCT-1),Multiple copies T-cell malignancies, Mcts1,MCT 1,MCT-1; MCT1; malignant T-cell-amplified sequence 1; multiple copies T-cell malignancies; malignant T cell amplified sequence 1
Search name
Rat Malignant T cell-amplified sequence 1(MCT-1) ELISA KIT ,Rat Multiple copies T-cell malignancies ELISA KIT ,Rat Mcts1 ELISA KIT ,Rat MCT 1 ELISA KIT ,Rat MCT-1 ELISA KIT ,Rat MCT1 ELISA KIT ,Rat malignant T-cell-amplified sequence 1 ELISA KIT ,Rat multiple copies T-cell malignancies ELISA KIT ,Rat malignant T cell amplified sequence 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat malignant t cell-amplified sequence 1,MCT-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCT-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MCT-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MCT-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MCT-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.