Rat Metallothionein-1,MT-1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MT1A,MT1;MT, MT1S; MTC; MT-1A; MT-IA; metallothionein 1A ,metallothionein 1S; metallothionein-IA; metallothionein 1A, metallothionein

Search name

Rat MT1A ELISA KIT , Rat MT ELISA KIT ,Rat MT1 ELISA KIT , Rat metallothionein ELISA KIT Rat MT1S ELISA KIT ,Rat MTC ELISA KIT ,Rat MT-1A ELISA KIT ,Rat MT-IA ELISA KIT ,Rat metallothionein 1A  ELISA KIT ,Rat metallothionein 1S ELISA KIT ,Rat metallothionein-IA ELISA KIT ,Rat metallothionein 1A ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat metallothionein-1,MT-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to MT-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MT-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MT-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MT-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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