Rat Methionine Adenosyltransferase I, MAT1A ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MAT1A,MAT; MATA1; SAMS; SAMS1; MAT 1; MAT-I/III; S-adenosylmethionine synthase isoform type-1; S-adenosylmethionine synthetase isoform type-1; adoMet synthase 1; adoMet synthetase 1; methionine adenosyltransferase 1; methionine adenosyltransferase I/III; methionine adenosyltransferase I alpha

Search name

Rat MAT1A ELISA KIT ,Rat MAT ELISA KIT ,Rat MATA1 ELISA KIT ,Rat SAMS ELISA KIT ,Rat SAMS1 ELISA KIT ,Rat MAT 1 ELISA KIT ,Rat MAT-I/III ELISA KIT ,Rat S-adenosylmethionine synthase isoform type-1 ELISA KIT ,Rat S-adenosylmethionine synthetase isoform type-1 ELISA KIT ,Rat adoMet synthase 1 ELISA KIT ,Rat adoMet synthetase 1 ELISA KIT ,Rat methionine adenosyltransferase 1 ELISA KIT ,Rat methionine adenosyltransferase I/III ELISA KIT ,Rat methionine adenosyltransferase I alpha ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat s-adenosylmethionine synthase isoform type-1,AdoMet synthase 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to AdoMet synthase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AdoMet synthase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AdoMet synthase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AdoMet synthase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF Manual download