Rat MORN4 ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name MORN4
Protein Name MORN repeat-containing protein 4
Alternative Name
Rat MORN4 ELISA KIT, Rat C10orf83 ELISA KIT ,Rat UTA ELISA KIT ,Rat bA548K23.4 ELISA KIT ,Rat rtp ELISA KIT ,Rat MORN repeat-containing protein 4 ELISA KIT ,Rat 44050 protein ELISA KIT ,Rat protein 44050 ELISA KIT ,Rat retinophilin homolog ELISA KIT ,Rat MORN repeat containing 4 ELISA KIT
Intended use
Rat MORN4 ELISA KIT allows for the in vitro quantitative determination of MORN4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Reagent | Quantity |
Assay plate | 1 |
Standard | 2 |
Sample Diluent | 1 × 20mL |
Assay Diluent A | 1 × 10mL |
Assay Diluent B | 1 × 10mL |
Detection Reagent A | 1 × 120μL |
Detection Reagent B | 1 × 120μL |
Wash Buffer(25 x concentrate) | 1 × 30mL |
Substrate | 1 × 10mL |
Stop Solution | 1 × 10mL |
Plate sealer | 5 |
Test principle
The microtiter plate provided in Rat MORN4 ELISA KIT has been pre-coated with an MORN4 antibody specific to MORN4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MORN4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MORN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MORN4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20