Rat Major histocompatibility complex class I- related gene protein, MR1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MR1,major histocompatibility complex class I-related gene protein; HLALS; MHC class I-like antigen MR-1; MHC class I-related gene protein; MHC class-I related-gene protein; class I histocompatibility antigen-like protein; major histocompatibility complex, class I-like sequence; major histocompatibility complex, class I-related

Search name

Rat MR1 ELISA KIT ,Rat major histocompatibility complex class I-related gene protein ELISA KIT ,Rat HLALS ELISA KIT ,Rat MHC class I-like antigen MR-1 ELISA KIT ,Rat MHC class I-related gene protein ELISA KIT ,Rat MHC class-I related-gene protein ELISA KIT ,Rat class I histocompatibility antigen-like protein ELISA KIT ,Rat major histocompatibility complex ELISA KIT ,Rat  class I-like sequence ELISA KIT ,Rat major histocompatibility complex ELISA KIT ,Rat class I-related ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat major histocompatibility complex class i-related gene protein,Major histocompatibility complex class I-related gene protein concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major histocompatibility complex class I-related gene protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Major histocompatibility complex class I-related gene protein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Major histocompatibility complex class I-related gene protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Major histocompatibility complex class I-related gene protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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