Rat Adaptin ear- binding coat- associated protein 1, NECAP1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

NECAP1,NECAP endocytosis-associated protein 1; NECAP-1; adaptin ear-binding coat-associated protein 1; adaptin-ear-binding coat-associated protein 1; NECAP endocytosis associated 1

Search name

Rat NECAP1 ELISA KIT ,Rat NECAP endocytosis-associated protein 1 ELISA KIT ,Rat NECAP-1 ELISA KIT ,Rat adaptin ear-binding coat-associated protein 1 ELISA KIT ,Rat adaptin-ear-binding coat-associated protein 1 ELISA KIT ,Rat NECAP endocytosis associated 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Adaptin ear- binding coat- associated protein 1, NECAP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to NECAP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NECAP1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NECAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NECAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.