Rat Neurofilament light polypeptide, NEFL ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

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Intended use

Rat NEFL ELISA KIT allows for the in vitro quantitative determination of rat neurofilament light polypeptide concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Rat NEFL ELISA KIT has been pre-coated with an antibody specific to NEFL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NEFL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NEFL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NEFL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Rat NEFL ELISA KIT Range 15.6-1000pg/ml

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