Rat Neuropilin 1, NRP1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

NRP1,neuropilin 1,BDCA4; CD304; NP1; NRP; VEGF165R; neuropilin-1; transmembrane receptor; vascular endothelial cell growth factor 165 receptor

Search name

Rat NRP1 ELISA KIT ,Rat neuropilin 1 ELISA KIT ,Rat BDCA4 ELISA KIT ,Rat CD304 ELISA KIT ,Rat NP1 ELISA KIT ,Rat NRP ELISA KIT ,Rat VEGF165R ELISA KIT ,Rat neuropilin-1 ELISA KIT ,Rat transmembrane receptor ELISA KIT ,Rat vascular endothelial cell growth factor 165 receptor ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat neuropilin-1,Neuropilin-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropilin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Neuropilin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neuropilin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neuropilin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.