Rat NLR family CARD domain- containing protein 4, NLRC4 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
NLRC4,AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF; CARD, Clan protein, LRR and NACHT-containing protein; ICE-protease activating factor; NLR family CARD domain-containing protein 4; NOD-like receptor C4; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4; NLR family CARD domain containing 4
Search name
Rat NLRC4 ELISA KIT,Rat AIFEC ELISA KIT,Rat CARD12 ELISA KIT,Rat CLAN ELISA KIT,Rat CLAN1 ELISA KIT,Rat CLANA ELISA KIT,Rat CLANB ELISA KIT,Rat CLANC ELISA KIT,Rat CLAND ELISA KIT,Rat CLR2.1 ELISA KIT,Rat FCAS4 ELISA KIT,Rat IPAF ELISA KIT,Rat CARD ELISA KIT, Rat Clan protein ELISA KIT,Rat LRR and NACHT-containing protein ELISA KIT,Rat ICE-protease activating factor ELISA KIT,Rat NLR family CARD domain-containing protein 4 ELISA KIT,Rat NOD-like receptor C4 ELISA KIT,Rat caspase recruitment domain family member 12 ELISA KIT,Rat caspase recruitment domain-containing protein 12 ELISA KIT,Rat nucleotide-binding oligomerization domain ELISA KIT,Rat leucine rich repeat and CARD domain containing 4 ELISA KIT,Rat NLR family CARD domain containing 4 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat NLR family CARD domain-containing protein 4, NLRC4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLRC4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NLRC4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NLRC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NLRC4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.