Rat ORM1- like protein 3, ORMDL3 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Ormdl3,ORM1-like protein 3; ORM1-like 3 (S. cerevisiae)

Search name

Rat Ormdl3 ELISA KIT ,Rat ORM1-like protein 3 ELISA KIT ,Rat ORM1-like 3 S. cerevisiae ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat ORM1- like protein 3, ORMDL3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to ORM1- like protein 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ORM1- like protein 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ORM1- like protein 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ORM1- like protein 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.