Rat Pendrin, Slc26a4 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Pendrin,Sodium-independent chloride/iodide transporter,Solute carrier family 26 member 4, SLC26A4,pendrin; DFNB4; EVA; PDS; TDH2B; sodium-independent chloride/iodide transporter; solute carrier family 26, member 4; solute carrier family 26member 4, anion exchanger member 4, Cfex,Pat1,PDS
Search name
Rat Pendrin ELISA KIT ,Rat Sodium-independent chloride/iodide transporter ELISA KIT ,Rat Solute carrier family 26 member 4 ELISA KIT ,Rat SLC26A4 ELISA KIT ,Rat pendrin ELISA KIT ,Rat DFNB4 ELISA KIT ,Rat EVA ELISA KIT ,Rat PDS ELISA KIT ,Rat TDH2B ELISA KIT ,Rat sodium-independent chloride/iodide transporter ELISA KIT ,Rat solute carrier family 26 member 4 ELISA KIT ,Rat solute carrier family 26member 4 ELISA KIT ,Rat anion exchanger member 4 ELISA KIT ,Rat Cfex ELISA KIT ,Rat Pat1 ELISA KIT ,Rat PDS ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat pendrin,Pendrin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pendrin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pendrin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pendrin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pendrin in the samples is then determined by comparing the O.D. of the samples to the standard curve.