Rat Phosphatidylinositide phosphatase SAC1, SACM1L ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

SACM1L,SAC1; phosphatidylinositide phosphatase SAC1; suppressor of actin 1; suppressor of actin mutations 1-like protein; SAC1 suppressor of actin mutations 1-like (yeast)

Search name

Rat SACM1L ELISA KIT ,Rat SAC1 ELISA KIT ,Rat phosphatidylinositide phosphatase SAC1 ELISA KIT ,Rat suppressor of actin 1 ELISA KIT ,Rat suppressor of actin mutations 1-like protein ELISA KIT ,Rat SAC1 suppressor of actin mutations 1-like ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Phosphatidylinositide phosphatase SAC1, SACM1L concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SACM1L. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SACM1L and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SACM1L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SACM1L in the samples is then determined by comparing the O.D. of the samples to the standard curve.