Rat Podocalyxin, Podxl ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Podocalyxin,Podocalyxin-like protein 1,PC,PCLP-1,PODXL,Gp200; PCLP; PCLP-1; GCTM-2 antigen; podocalyxin; podocalyxin-like protein 1; podocalyxin-like

Search name

Rat Podocalyxin ELISA KIT ,Rat Podocalyxin-like protein 1 ELISA KIT ,Rat PC ELISA KIT ,Rat PCLP-1 ELISA KIT ,Rat PODXL ELISA KIT ,Rat Gp200 ELISA KIT ,Rat PCLP ELISA KIT ,Rat PCLP-1 ELISA KIT ,Rat GCTM-2 antigen ELISA KIT ,Rat podocalyxin ELISA KIT ,Rat podocalyxin-like protein 1 ELISA KIT ,Rat podocalyxin-like ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat podocalyxin,Podocalyxin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Podocalyxin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Podocalyxin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Podocalyxin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Podocalyxin in the samples is then determined by comparing the O.D. of the samples to the standard curve.