Rat RT1-Bb ELISA Kit
FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Rat RT1-Bb ELISA KIT ,Rat RT1.B ELISA KIT ,Rat rano class II histocompatibility antigen ELISA KIT ,Rat  B-1 beta chain ELISA KIT ,Rat MHC class II antigen B beta chain ELISA KIT ,Rat RT1 class II histocompatibility antigen ELISA KIT ,Rat  B-1 beta chain ELISA KIT ,Rat RT1.B-beta(1) ELISA KIT ,Rat RT1.Bbeta 1 ELISA KIT ,Rat RT1 class II ELISA KIT ,Rat  locus Bb ELISA KIT

Intended use

Rat RT1-Bb ELISA kit allows for the in vitro quantitative determination of Rat RT1-Bb concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Rat RT1-Bb ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RT1-Bb and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RT1-Bb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RT1-Bb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Rat RT1-Bb ELISA kit  Range 0.15-10ng/ml

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