Rat Septin- 7, SEPT7 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

SEPT7,CDC10; CDC3; NBLA02942; SEPT7A; CDC10 (cell division cycle 10, S. cerevisiae, homolog); CDC10 protein homolog; septin-7; septin 7

Search name

Rat SEPT7 ELISA KIT ,Rat CDC10 ELISA KIT ,Rat CDC3 ELISA KIT ,Rat NBLA02942 ELISA KIT ,Rat SEPT7A ELISA KIT ,Rat CDC10 (cell division cycle 10 ELISA KIT ,Rat  S. cerevisiae ELISA KIT ,Rat  homolog) ELISA KIT ,Rat CDC10 protein homolog ELISA KIT ,Rat septin-7 ELISA KIT ,Rat septin 7 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Septin- 7, SEPT7 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEPT7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SEPT7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SEPT7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SEPT7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product