Rat Serpina3l ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name Serpina3l
Protein Name Serine protease inhibitor A3L
Alternative Name
Rat Serpina3l ELISA KIT ,Rat LOC299282 ELISA KIT ,Rat serine protease inhibitor A3L ELISA KIT ,Rat Ab1-021 ELISA KIT ,Rat CPi-23 ELISA KIT ,Rat SPI1 ELISA KIT ,Rat Spin1 ELISA KIT ,Rat Spin2a ELISA KIT ,Rat Serine protease inhibitor ELISA KIT ,Rat SPI-1 ELISA KIT ,Rat SPI-2.1 ELISA KIT ,Rat contrapsin-like protease inhibitor 3 ELISA KIT ,Rat contrapsin-like protease inhibitor-related protein ELISA KIT ,Rat liver regeneration protein lrryan ELISA KIT ,Rat serine protease inhibitor 1 ELISA KIT ,Rat serine protease inhibitor 2.1 ELISA KIT ,Rat serine protease inhibitor 2a ELISA KIT ,Rat serpin A3L ELISA KIT
Rat Serpina3l ELISA Kit Range 0.15-10ng/ml
Intended use
Rat Serpina3l ELISA KIT allows for the in vitro quantitative determination of Serpina3l concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20mL |
| Assay Diluent A | 1 × 10mL |
| Assay Diluent B | 1 × 10mL |
| Detection Reagent A | 1 × 120μL |
| Detection Reagent B | 1 × 120μL |
| Wash Buffer(25 x concentrate) | 1 × 30mL |
| Substrate | 1 × 10mL |
| Stop Solution | 1 × 10mL |
| Plate sealer | 5 |
Test principle
The microtiter plate provided in Rat Serpina3l ELISA KIT has been pre-coated with an Serpina3l antibody specific to Serpina3l. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Serpina3l and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serpina3l, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serpina3l in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20
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