Rat thrombin- antithrombin complex, TAT ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TATC,Thrombin-antithrombin complex, Thrombin-antithrombin,TAT, Thrombin, antithrombin
Search name
Rat TATC ELISA KIT ,Rat Thrombin-antithrombin complex ELISA KIT ,Rat Thrombin-antithrombin ELISA KIT ,Rat TAT ELISA KIT ,Rat Thrombin ELISA KIT ,Rat antithrombin ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Thrombin-antithrombin complex concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombin-antithrombin complex. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Thrombin-antithrombin complex and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Thrombin-antithrombin complex, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Thrombin-antithrombin complex in the samples is then determined by comparing the O.D. of the samples to the standard curve.