Rat tissue inhibitors of metalloproteinase 2, TIMP2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

TIMP2,TIMP-2,CSC-21K; DDC8; metalloproteinase inhibitor 2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2; TIMP metallopeptidase inhibitor 2,Metalloproteinase inhibitor 2,CSC-21K,Tissue inhibitor of metalloproteinases 2

Search name

Rat TIMP2 ELISA KIT ,Rat TIMP-2 ELISA KIT ,Rat CSC-21K ELISA KIT ,Rat DDC8 ELISA KIT ,Rat metalloproteinase inhibitor 2 ELISA KIT ,Rat tissue inhibitor of metalloproteinase 2 ELISA KIT ,Rat tissue inhibitor of metalloproteinases 2 ELISA KIT ,Rat TIMP metallopeptidase inhibitor 2 ELISA KIT ,Rat Metalloproteinase inhibitor 2 ELISA KIT ,Rat CSC-21K ELISA KIT ,Rat Tissue inhibitor of metalloproteinases 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Metalloproteinase inhibitor 2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metalloproteinase inhibitor 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Metalloproteinase inhibitor 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Metalloproteinase inhibitor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Metalloproteinase inhibitor 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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