Rat Transforming growth factor beta 2,TGFb2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Transforming growth factor beta-2,TGF-beta-2,Polyergin,Glioblastoma-derived T-cell suppressor factor,G-TSF,BSC-1 cell growth inhibitor,Cetermin,TGFB2,LDS4; TGF-beta2; BSC-1 cell growth inhibitor; G-TSF; cetermin; glioblastoma-derived T-cell suppressor factor; polyergin; prepro-transforming growth factor beta-2; transforming growth factor beta-2; transforming growth factor beta 2

Search name

Rat Transforming growth factor beta-2 ELISA KIT ,Rat TGF-beta-2 ELISA KIT ,Rat Polyergin ELISA KIT ,Rat Glioblastoma-derived T-cell suppressor factor ELISA KIT ,Rat G-TSF ELISA KIT ,Rat BSC-1 cell growth inhibitor ELISA KIT ,Rat Cetermin ELISA KIT ,Rat TGFB2 ELISA KIT ,Rat LDS4 ELISA KIT ,Rat TGF-beta2 ELISA KIT ,Rat BSC-1 cell growth inhibitor ELISA KIT ,Rat G-TSF ELISA KIT ,Rat cetermin ELISA KIT ,Rat glioblastoma-derived T-cell suppressor factor ELISA KIT ,Rat polyergin ELISA KIT ,Rat prepro-transforming growth factor beta-2 ELISA KIT ,Rat transforming growth factor beta-2 ELISA KIT ,Rat transforming growth factor beta 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat transforming growth factor beta-2,TGF-beta-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-beta-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TGF-beta-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TGF-beta-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TGF-beta-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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