Rat Transmembrane protein 126A, TMEM126A ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Transmembrane protein 126A, TMEM126A,OPA7

Search name

Rat TMEM126A ELISA Kit, Rat Transmembrane protein 126A ELISA Kit, Rat OPA7 ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Transmembrane protein 126A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane protein 126A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Transmembrane protein 126A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transmembrane protein 126A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Transmembrane protein 126A in the samples is then determined by comparing the O.D. of the samples to the standard curve.