Rat Valine, VAL ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Valine, 2-amino-3-methylbutanoic acid,Val,V

Search name
Rat Valine ELISA Kit, Rat 2-amino-3-methylbutanoic acid ELISA Kit, Rat V ELISA Kit, Rat VAL ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Valine concentrations in serum, plasma, tissue homogenates,
cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Valine, During the reaction, Valine in the sample or standard competes with a fixed amount of
biotin-labeled Valine for sites on a pre-coated Monoclonal antibody specific to Valine. Excess conjugate and unbound sample or standard
are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Valine in the
samples is then determined by comparing the O.D. of the samples to the standard curve.

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