Rat Voltage- dependent anion- selective channel protein 1, VDAC1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

VDAC1,VDAC,PORIN; VDAC-1; outer mitochondrial membrane protein porin 1; plasmalemmal porin; porin 31HL; porin 31HM; voltage-dependent anion-selective channel protein 1; voltage-dependent anion channel 1

Search name

Rat VDAC1 ELISA KIT ,Rat VDAC ELISA KIT ,Rat PORIN ELISA KIT ,Rat VDAC-1 ELISA KIT ,Rat outer mitochondrial membrane protein porin 1 ELISA KIT ,Rat plasmalemmal porin ELISA KIT ,Rat porin 31HL ELISA KIT ,Rat porin 31HM ELISA KIT ,Rat voltage-dependent anion-selective channel protein 1 ELISA KIT ,Rat voltage-dependent anion channel 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Voltage- dependent anion- selective channel protein 1, VDAC1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to VDAC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for VDAC1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain VDAC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VDAC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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