Rat Vascular endothelial growth factor receptor 2, VEGFR2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Vascular endothelial growth factor receptor ,VEGFR-2,Protein-tyrosine kinase receptor flk-1,Fetal liver kinase ,FLK-1,Kinase insert domain receptor, KDR, VEGFR-2,CD309; FLK1; VEGFR; VEGFR2; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; soluble VEGFR2; tyrosine kinase growth factor receptor; kinase insert domain receptor (a type III receptor tyrosine kinase),  Kinase NYK, Protein-tyrosine kinase receptor flk-1

Search name

Rat Vascular endothelial growth factor receptor ELISA KIT ,Rat VEGFR-2 ELISA KIT ,Rat Protein-tyrosine kinase receptor flk-1 ELISA KIT ,Rat Fetal liver kinase  ELISA KIT ,Rat FLK-1 ELISA KIT ,Rat Kinase insert domain receptor ELISA KIT ,Rat KDR ELISA KIT ,Rat VEGFR-2 ELISA KIT ,Rat CD309 ELISA KIT ,Rat FLK1 ELISA KIT ,Rat VEGFR ELISA KIT ,Rat VEGFR2 ELISA KIT ,Rat fetal liver kinase 1 ELISA KIT ,Rat fetal liver kinase-1 ELISA KIT ,Rat protein-tyrosine kinase receptor Flk-1 ELISA KIT ,Rat soluble VEGFR2 ELISA KIT ,Rat tyrosine kinase growth factor receptor ELISA KIT ,Rat kinase insert domain receptor  ELISA KIT ,Rat a type III receptor tyrosine kinase ELISA KIT,Rat Kinase NYK ELISA KIT, Rat Protein-tyrosine kinase receptor flk-1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat vascular endothelial growth factor receptor 2,VEGFR-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGFR-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for VEGFR-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain VEGFR-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VEGFR-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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