Rat vinculin, VCL ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Vinculin,Metavinculin,CMD1W; CMH15; MVCL, VCL,

Search name
Rat Vinculin ELISA Kit, Rat VCL ELISA Kit, Rat Metavinculin ELISA Kit, Rat CMD1W ELISA Kit, Rat CMH15
ELISA Kit, Vinculin ELISA Kit, VCL ELISA Kit, Metavinculin ELISA Kit, CMD1W ELISA Kit, CMH15 ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat vinculin,Vinculin concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vinculin. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Vinculin and Avidin conjugated
to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain Vinculin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Vinculin in the samples is then determined by comparing
the O.D. of the samples to the standard curve.