Rat Smarcal1 ELISA Kit
This Sandwich ELISA kit provides quantitative detection of target protein in Rattus norvegicus (Rat) biological samples including serum, plasma, tissue homogenates and other biological fluids. The assay delivers a detection range of with high sensitivity of , enabling precise measurement across physiological and pathological concentrations. Optimized antibody pairs ensure specificity and reproducibility for research applications in immunology, biochemistry, and biomarker studies. Validated performance characteristics support reliable quantification in preclinical models.
What is protein?
is a biologically active molecule encoded by the target gene in Rattus. This protein plays significant roles in various physiological processes and has been identified as an important research target in biomedical studies. The protein's function, expression patterns, and regulatory mechanisms have been extensively documented in peer-reviewed literature. Researchers investigate this target to understand its involvement in cellular signaling pathways, disease mechanisms, and potential therapeutic applications. The molecular weight, structural features, and post-translational modifications of this protein contribute to its biological activity and interaction with other molecules in biochemical networks.
How does the Sandwich ELISA principle work?
The Sandwich ELISA method employs a two-step immunoassay approach for target detection. In this format, a capture antibody specific to the target protein is immobilized on the microplate surface. When samples containing the target are added, the antigen binds to the capture antibody. A detection antibody conjugated to an enzyme (typically horseradish peroxidase) then binds to a different epitope on the captured target. After washing away unbound components, a chromogenic substrate (TMB) is added. The enzyme catalyzes a color change proportional to the amount of bound target. The reaction is stopped with acid solution, and absorbance is measured at 450nm. Concentration values are calculated by interpolation from a standard curve generated using known concentrations of recombinant protein.
Which sample types are compatible?
This ELISA kit has been validated for use with multiple biological sample types including serum, plasma, tissue homogenates, and cell culture supernatants. For serum collection, allow blood samples to clot at room temperature for 30 minutes, then centrifuge at 2000-3000g for 10 minutes. Plasma requires collection with anticoagulants such as EDTA, heparin, or sodium citrate, followed by centrifugation within 30 minutes of collection. Tissue homogenates should be prepared in ice-cold PBS at a ratio of 1:9 (w/v), followed by sonication or mechanical disruption and centrifugation to collect the supernatant. Cell culture supernatants should be collected after appropriate incubation periods, clarified by centrifugation, and stored appropriately. All samples should be tested immediately or aliquoted and stored at -20??C or below to prevent degradation.
What are storage requirements?
The complete kit should be stored at 2-8??C upon receipt until the expiration date printed on the label. The unopened kit remains stable for 12 months when stored properly. After opening, individual components have varying stability: the microplate (coated with antibody) should be stored desiccated at 2-8??C for up to 1 month; standards and detection reagents should be kept at 2-8??C; the conjugate solution may require storage at 2-8??C or freezing depending on formulation. Avoid repeated freeze-thaw cycles for any component. Protect light-sensitive reagents from direct exposure. Always check individual component labels for specific storage instructions. Improper storage conditions may affect assay performance and invalidate results.
What are the quality control measures for this target ELISA kit?
This ELISA kit undergoes rigorous quality control testing during manufacturing. Each lot is validated for sensitivity, specificity, precision, accuracy, and parallelism. Intra-assay precision is verified with CV values below 8%, while inter-assay precision maintains CV below 10%. Recovery rates between 85-115% confirm accuracy across the detection range. Dilution linearity is confirmed through serial dilution experiments showing correlation coefficients above 0.99. Cross-reactivity testing ensures minimal interference from structurally similar molecules. Standard curves are generated with each run using recombinant protein calibrators traceable to international reference materials when available. Batch-to-batch consistency is monitored through statistical process control methods.
Research Use Only: This product is intended for laboratory research purposes only. Not for diagnostic or therapeutic use.
For technical support and ordering information, please contact our scientific support team.
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