Bovine Methionyl- tRNA formyltransferase, mitochondrial, MTFMT ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MTFMT,COXPD15; FMT1; methionyl-tRNA formyltransferase, mitochondrial; mitochondrial methionyl-tRNA formyltransferase

Search name

Bovine MTFMT ELISA KIT ,Bovine COXPD15 ELISA KIT ,Bovine FMT1 ELISA KIT ,Bovine methionyl-tRNA formyltransferase mitochondrial ELISA KIT ,Bovine mitochondrial methionyl-tRNA formyltransferase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Methionyl- tRNA formyltransferase, mitochondrial, MTFMT concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionyl- tRNA formyltransferase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Methionyl- tRNA formyltransferase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Methionyl- tRNA formyltransferase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Methionyl- tRNA formyltransferase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product

Delivery time 4-6month