Bovine Sex determining region Y protein, SRY ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Sex-determining region Y protein,Testis-determining factor, SRY,SRXX1; SRXY1; TDF; TDY; essential protein for sex determination in Bovine males; sex-determining region Y protein; sex-determining region on Y; testis-determining factor on Y; sex determining region Y

Search name

Bovine Sex-determining region Y protein ELISA KIT ,Bovine Testis-determining factor ELISA KIT ,Bovine SRY ELISA KIT ,Bovine SRXX1 ELISA KIT ,Bovine SRXY1 ELISA KIT ,Bovine TDF ELISA KIT ,Bovine TDY ELISA KIT ,Bovine essential protein for sex determination in Bovine males ELISA KIT ,Bovine sex-determining region Y protein ELISA KIT ,Bovine sex-determining region on Y ELISA KIT ,Bovine testis-determining factor on Y ELISA KIT ,Bovine sex determining region Y ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine sex-determining region y protein,SRY concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SRY. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SRY and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SRY, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SRY in the samples is then determined by comparing the O.D. of the samples to the standard curve.