Bovine Stress-induced-phosphoprotein 1, STI1 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

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Intended use

Bovine STI1 ELISA kit allows for the in vitro quantitative determination of Bovine STI1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Bovine STI1 ELISA kit has been pre-coated with an antibody specific to STI1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for STI1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain STI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of STI1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Bovine STI1 ELISA kit Manual Request

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