Canine Matrix metalloproteinase- 9, MMP9 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Matrix metalloproteinase-9,MMP-9,Gelatinase B,GELB,92 kDa gelatinase,92 kDa type IV collagenase,

Search name
Canine MMP9 ELISA Kit,Canine Matrix metalloproteinase-9 ELISA Kit, Canine MMP-9 ELISA Kit,Matrix metalloproteinase-9 ELISA Kit

Intended use
Canine MMP9 ELISA Kit allows for the in vitro quantitative determination of Canine matrix metalloproteinase-9,MMP9 concentrations in serum,Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in Canine MMP9 ELISA Kit has been pre-coated with an antibody specific to MMP9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MMP-9 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.Only those wells that contain MMP9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MMP9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Canine MMP9 ELISA Kit Range 0.31-20mg/ml

Canine MMP9 ELISA Kit PDF manual download

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