Human ADP- ribosylation factor- like protein 9, ARL9 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ARL9,ADP-ribosylation factor-like 9; ADP-ribosylation factor like GTPase 9

Search name

Human ARL9 ELISA KIT, Human ADP-ribosylation factor-like 9 ELISA KIT, Human ADP-ribosylation factor like GTPase 9 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human ADP- ribosylation factor- like protein 9, ARL9 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADP-ribosylation factor-like protein 9,ARL9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human ADP-ribosylation factor-like protein 9,ARL9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human ADP-ribosylation factor-like protein 9,ARL9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human ADP-ribosylation factor-like protein 9,ARL9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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