Human Dynein heavy chain 3, axonemal, DNAH3 ELISA KIT
Product Name:Human Dynein heavy chain 3, axonemal, DNAH3 ELISA KIT
Packing:96T
Catalog No.:ELI-48128h
Gene Name:DNAH3
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Dynein heavy chain 3(axonemal(DNAH3
Alternative Name:DNAH3,Dynein heavy chain 3(axonemal(DNAH3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Dynein heavy chain 3, axonemal, DNAH3 ELISA KIT allows for the in vitro quantitative determination of Dynein heavy chain 3(axonemal(DNAH3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Dynein heavy chain 3, axonemal, DNAH3 ELISA KIT has been pre-coated with an Dynein heavy chain 3(axonemal(DNAH3 antibody specific to Dynein heavy chain 3(axonemal(DNAH3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Dynein heavy chain 3(axonemal(DNAH3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dynein heavy chain 3(axonemal(DNAH3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Dynein heavy chain 3(axonemal(DNAH3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!