Human Follistatin-related protein 1, FSTL1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Follistatin-related protein 1,Follistatin-like protein 1,FSTL1,FRP; FSL1; MIR198; follistatin-like protein 1; follistatin-related protein 1; follistatin-like 1,TGF-beta-inducible protein TSC-36

Search name

Human Follistatin-related protein 1 ELISA KIT ,Human Follistatin-like protein 1 ELISA KIT ,Human FSTL1 ELISA KIT ,Human FRP ELISA KIT ,Human FSL1 ELISA KIT ,Human MIR198 ELISA KIT ,Human follistatin-like protein 1 ELISA KIT ,Human follistatin-related protein 1 ELISA KIT ,Human follistatin-like 1 ELISA KIT ,Human TGF-beta-inducible protein TSC-36 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human follistatin-related protein 1,FSTL1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to FSTL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FSTL1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FSTL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FSTL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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