Human Indian Hedgehog Homolog, IHH ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Human IHH ELISA KIT ,Human BDA1 ELISA KIT ,Human HHG2 ELISA KIT ,Human indian hedgehog protein ELISA KIT ,Human Indian hedgehog homolog ELISA KIT ,Human indian hedgehog ELISA KIT

Intended use

Human IHH ELISA kit allows for the in vitro quantitative determination of Human IHH concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Human IHH ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IHH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IHH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human IHH ELISA kit Range 0.15-10ng/ml

Human IHH ELISA kit Manual

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