Human Kelch- like protein 23, KLHL23 ELISA KIT
Product Name:Human Kelch- like protein 23, KLHL23 ELISA KIT
Packing:96T
Catalog No.:ELI-46401h
Gene Name:KLHL23
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Kelch-like protein 23(KLHL23
Alternative Name:KLHL23,Kelch-like protein 23(KLHL23
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Kelch- like protein 23, KLHL23 ELISA KIT allows for the in vitro quantitative determination of Kelch-like protein 23(KLHL23 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Kelch- like protein 23, KLHL23 ELISA KIT has been pre-coated with an Kelch-like protein 23(KLHL23 antibody specific to Kelch-like protein 23(KLHL23 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Kelch-like protein 23(KLHL23 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Kelch-like protein 23(KLHL23, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Kelch-like protein 23(KLHL23 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!