Human POMT1 ELISA Kit

Product Name:Human POMT1 ELISA Kit
Packing:96T

Catalog No.:ELA-E1172h

Gene Name:POMT1

Detect Range:0.78-50 ng/mL

Sensitivity:0.22ng/mL

Target Protein Name:Human Protein O-mannosyl-transferase 1

Alternative Name:Protein O-mannosyl-transferase 1, Dolichyl-phosphate-mannose--protein mannosyltransferase 1, POMT1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human POMT1 ELISA Kit allows for the in vitro quantitative determination of Human Protein O-mannosyl-transferase 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human POMT1 ELISA Kit has been pre-coated with an POMT1 antibody specific to Human Protein O-mannosyl-transferase 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Protein O-mannosyl-transferase 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Protein O-mannosyl-transferase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm