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Catalog EF005672
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
TMPPE ELISA KIT Sensitivity< 0.188ng/ml
Application TMPPE ELISA KIT|Human is for detecting the concentration of Human TMPPE in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of TMPPE ELISA KIT|Human
The microtiter plate provided in TMPPE ELISA KIT|Human has been pre-coated with an Human TMPPE antibody specific to Human TMPPE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human TMPPEantibody preparation specific for Human TMPPE then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human TMPPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human TMPPEin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that TMPPE ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!