Mouse Collagen alpha- 2(I) chain, Col1a2 ELISA KIT

Product Name:Mouse Collagen alpha- 2(I) chain, Col1a2 ELISA KIT
Packing:96T

Catalog No.:ELI-51022m

Gene Name:Mouse Col1a2

Detect Range:15.62-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Mouse Col1a2

Alternative Name:Mouse Collagen alpha-2(I) chain, Col1a2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Collagen alpha- 2(I) chain, Col1a2 ELISA KIT allows for the in vitro quantitative determination of Mouse Col1a2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Collagen alpha- 2(I) chain, Col1a2 ELISA KIT has been pre-coated with an Mouse Collagen alpha-2(I) chain, Col1a2 antibody specific to Mouse Col1a2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Col1a2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Col1a2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Col1a2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!