Mouse Kallikrein 1- related peptidase b5, Klk1b5 ELISA KIT

Product Name:Mouse Kallikrein 1- related peptidase b5, Klk1b5 ELISA KIT
Packing:96T

Catalog No.:ELI-08069m

Gene Name:Klk1b5

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Mouse Kallikrein 1- related peptidase b5

Alternative Name:Klk1b5,Mouse Kallikrein 1- related peptidase b5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Kallikrein 1- related peptidase b5, Klk1b5 ELISA KIT allows for the in vitro quantitative determination ofMouse Kallikrein 1- related peptidase b5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Kallikrein 1- related peptidase b5, Klk1b5 ELISA KIT has been pre-coated with an Klk1b5 antibody specific to Mouse Kallikrein 1- related peptidase b5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Kallikrein 1- related peptidase b5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Kallikrein 1- related peptidase b5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Kallikrein 1- related peptidase b5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!